Expression of hSef and FGF-2 in epithelial ovarian tumor.
- Author:
Quan-ling FENG
1
;
Hui-rong SHI
;
Li-juan QIAO
;
Jing ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Cystadenocarcinoma, Mucinous; genetics; metabolism; pathology; surgery; Cystadenocarcinoma, Serous; genetics; metabolism; pathology; surgery; Cystadenoma, Mucinous; genetics; metabolism; pathology; surgery; Cystadenoma, Serous; genetics; metabolism; pathology; surgery; Female; Fibroblast Growth Factor 2; genetics; metabolism; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Middle Aged; Ovarian Neoplasms; genetics; metabolism; pathology; surgery; Ovary; metabolism; RNA, Messenger; metabolism; Real-Time Polymerase Chain Reaction; Receptors, Interleukin; genetics; metabolism
- From: Chinese Journal of Oncology 2011;33(10):770-774
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of human similar expression to FGF gene(hSef) and fibroblast growth factor-2(FGF-2) and their correlation with epithelial ovarian tumor.
METHODSImmunohistochemical SP staining was used to detect the expression of hSef and FGF-2 proteins in 31 cases of epithelial ovarian carcinoma (EOC), 18 cases of benign epithelial tumor (BET), 10 cases of normal ovarian (NO) tissues collected from July 2007 to May 2008. The expression of hSef mRNA in 24 cases of EOC, BET and NO collected from July 2008 to May 2009 were analyzed by RT-PCR.
RESULTSThe results of immunohistochemical study showed that the expression of hSef in the EOC tissues were significantly lower than that in the NO and BET (P < 0.001). However, the expression of FGF-2 was higher (P = 0.002). The expression of hSef had a negative correlation with FGF-2 (r(s) = -0.324, P = 0.012). The RT-PCR results showed that there was a gradually declined trend of expression of hSef in NO, BET to EOC (P < 0.001), but the expression of FGF-2 in NO, BET to EOC was gradually increased (P < 0.001), with a significant negative correlation (NO: r(s) = -0.910, P < 0.001; BET: r(s) = -0.859, P < 0.001; EOC: r(s) = -0.888, P < 0.001).
CONCLUSIONSThe expression of hSef is decreased in epithelial ovarian carcinoma tissue, but the expression of FGF-2 is increased. It is likely that low hSef expression is related to the the carcinogenesis and development of epithelial ovarian carcinoma by suppressing the promoting effects of FGF-2 to cell proliferation.
