Measurement of differential values of inhaled volumes and exhaled volumes in mice.
- Author:
Wei-hua XU
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Exhalation; physiology; Inhalation; physiology; Mice; Plethysmography; Pressure; Temperature; Tidal Volume; Vital Capacity; physiology
- From: Journal of Zhejiang University. Medical sciences 2010;39(3):322-325
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differential value of inhaled volume and exhaled volume in anesthesia mice, and its effect on the pressure change inside of plethysmograph.
METHODSPressure and temperature in head chamber of double-chamber plethysmograph were tested when mice were placed in the body chamber. The differential values of inhaled volume and exhaled volume of mice were calculated.
RESULTThe baseline pressure in head chamber increased with temperature rising within 12 minutes, then went down though temperature remained high. The inhaled volumes of 8 mice were (0.2842 + or - 0.0173)ml,and the differential value of inhaled volume and exhaled volume was (0.0012 + or - 0.0002)ml.
CONCLUSIONThe inhaled volume is larger than exhaled volume, which makes pressure baseline of plethysmograph decreased. The differential value may be generated from respiratory quotient.
