Effects of Sema3A derived from tumor cells on functions of dendritic cells.
- Author:
Xie-lai ZHOU
1
;
Yin HUANG
;
Fang WANG
;
Ling-fei CAI
;
Li-huang ZHANG
;
Li-yun SHI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Dendritic Cells; drug effects; immunology; Female; Humans; Lung Neoplasms; immunology; metabolism; pathology; Male; Mice; Mice, Inbred C57BL; Semaphorin-3A; genetics; metabolism; pharmacology; Transfection; Tumor Escape; immunology
- From: Journal of Zhejiang University. Medical sciences 2010;39(4):364-369
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of tumor cell-derived Sema3A on the immunological functions of murine dendritic cells (DCs).
METHODSLung adenocarcinoma A549 cells were transfected with small interference RNA, Si-Sema and Si-mut, and the interference efficiency was determined by real-time PCR and Western-blot. The concentrated supernatants from cultured tumor cells, Si-Sema and Si-mut-infected tumor cells were subjected to DCs respectively. The immunophenotypes of DCs were analyzed by flow cytometry, the production of IL-12P70 and the ability of DCs to stimulate DO11. 10 T cells secreting IFN-gamma and IL-2 were detected by enzyme linked immunosorbent assay (ELISA).
RESULTSKnockdown with Si-Sema3A significantly decreased the secretion of Sema3A by A549 cells in comparison with the Si-mut cells. DCs exposed to supernatants from Si-Sema cells showed elevated levels of MHC, CD40 and CD80, more production of IL-12P70, and enhanced capability of activating antigen-specific T cells, as evidenced by the remarkably increased levels of IFN-gamma and IL-2.
CONCLUSIONA549 cells secrete Sema3A to inhibit the maturation and functions of DCs, which might be associated with the unidentified mechanism of immune evasion by tumor cells.