Effects of hydrogen gas inhalation on serum high mobility group box 1 levels in severe septic mice.
- Author:
Ke-Liang XIE
1
;
Li-Chao HOU
;
Guo-Lin WANG
;
Li-Ze XIONG
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Inhalation; Animals; Disease Models, Animal; HMGB1 Protein; blood; Hydrogen; administration & dosage; therapeutic use; Male; Mice; Mice, Inbred C57BL; Sepsis; blood; drug therapy
- From: Journal of Zhejiang University. Medical sciences 2010;39(5):454-457
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of hydrogen gas inhalation on survival rate and serum high mobility group box 1 (HMGB1) levels in severe septic mice.
METHODSSevere sepsis was induced by cecal ligation and puncture (CLP) operation in mice.A total of 248 mice were randomly divided into four groups: sham operation group (sham), sham operation with hydrogen gas inhalation group (sham+H2), severe CLP group (severe CLP) and severe CLP with hydrogen gas inhalation group (severe CLP+H2). Hydrogen gas inhalation was given for 1 h at 1st and 6th h after CLP or sham operation, respectively. The survival rates and serum HMGB1 levels of all groups at different time points were measured.
RESULTThe 7-d survival rates of severe CLP mice was 0 % (Compared with Sham group, P <0.05), and the serum HMBG1 levels from h2 to h32 after CLP operation were significantly increased in severe CLP mice (Compared with Sham group, P <0.05). Hydrogen gas treatment increased the 7-d survival rate of severe CLP mice to 60 % (Compared with severe sepsis group, P <0.05) and significantly reduced the serum HMGB1 levels at different time points (Compared with severe sepsis group, P <0.05).
CONCLUSIONHydrogen gas inhalation can decrease the serum HMGB1 levels and increase the survival rate of rats with severe sepsis.
