Effects of isoflurane, sevoflurane and desflurane on expression of ICAM-1 and VCAM-1 in LPS-induced rat lung microvascular endothelial cells.
- Author:
Xing LUO
1
;
Chang-Hong MIAO
;
Bao-Xue GE
;
Hao JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Anesthetics, Inhalation; administration & dosage; pharmacology; Animals; Cells, Cultured; Endothelial Cells; drug effects; metabolism; Endothelium, Vascular; cytology; Intercellular Adhesion Molecule-1; metabolism; Isoflurane; administration & dosage; analogs & derivatives; pharmacology; Lipopolysaccharides; pharmacology; Lung; blood supply; Methyl Ethers; administration & dosage; pharmacology; Rats; Rats, Sprague-Dawley; Vascular Cell Adhesion Molecule-1; metabolism
- From: Journal of Zhejiang University. Medical sciences 2010;39(5):464-469
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of isoflurane, sevoflurane and desflurane on the expression of ICAM-1 and VCAM-1 in LPS-induced rat lung microvascular endothelial cells (RLMVECs).
METHODSCultured LPS-treated RLMVECs were exposed to 0.7, 1.0 or 2.0 minimum alveolar concentration (MAC) iosoflurane, sevoflurane or desflurane in 6 h. The protein expression of ICAM-1 and VCAM-1 was determined by Western blot analysis. The expression of ICAM-1 mRNA was detected by reverse-transcription polymerase chain reaction (RT-PCR).
RESULTIsoflurane at concentration of 1.0 MAC up-regulated the expression of ICAM-1 in LPS-induced RLMVECs (P <0.05); while same concentrations of sevoflurane and desflurane down-regulated the expression of ICAM-1 (P<0.05). Desflurane at concentration of 2.0 MAC up-regulated the expression of ICAM-1 in non-LPS-induced RLMVECs. All the volatile anesthetics down-regulated the expression of VCAM-1 in a dose-dependent manner.
CONCLUSIONCompared to isoflurane, 1.0 MAC sevoflurane and desflurane down-regulate the expression of ICAM-1, which may be the molecule mechanism of their protective effect in acute lung injury.
