Construction of RegIα over-expression vector and its effect on proliferation and apoptosis of gastric cancer cells.
- Author:
Qun-Yan ZHOU
1
;
Xiao-Feng LU
;
Liang-Jing WANG
;
Jian-Min SI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; Genetic Vectors; Humans; Plasmids; genetics; Stomach Neoplasms; metabolism; pathology; Transfection
- From: Journal of Zhejiang University. Medical sciences 2010;39(5):499-505
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct RegIα over-expression vector and to evaluate the effect of RegIα on the proliferation and apoptosis of gastric cancer MKN28 cells in vitro.
METHODSFull sequence of RegIα cDNA was amplified from normal gastric tissue samples by RT-PCR and cloned into pIRES2-EGFP vector. RT-PCR and Western blot were performed to detect expression levels of RegIα in MKN28 cells. The effects of over-expression RegIα on cell proliferation was measured by MTT assay and apoptosis was detected by flow cytometry.
RESULTRegIα cDNA over-expression vector of pIRES2-RegIα-EGFP was successfully constructed. The expressions of RegIα in MKN28 cells, including mRNA and protein levels, were significantly increased after stable transfection, which resulted in cell proliferation and anti-apoptotic effect induced by H(2)O(2).
CONCLUSIONThe over-expression of RegIα can promote cell proliferation and reduce cell apoptosis when induced by H(2)O(2) in gastric cancer cells.
