Construction and identification of lentiviral vector for RNA interference targeting STUB1 gene.
- Author:
Hong ZHAO
1
;
Jing-yu ZHANG
;
Wan-hai XU
;
Zi-chao YANG
;
Qing-jie ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Gene Targeting; Genetic Vectors; Lentivirus; genetics; RNA Interference; RNA, Small Interfering; genetics; Ubiquitin-Protein Ligases; genetics
- From: Journal of Zhejiang University. Medical sciences 2010;39(6):623-627
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct and identification of a lentiviral vector for RNA interference (RNAi) targeting STUB1 gene.
METHODSA pair of complementary small hairpin RNA (shRNA) oligonucleotides targeting STUB1 gene was designed, synthesized and inserted into linearized pMagic 4.0 vector. The recombinant plasmid was identified by double restriction digestion with Age I/EcoR I and DNA sequencing.
RESULTPCR and DNA sequencing showed that the shRNA sequence was successfully inserted into pMagic 4.0 vector. The pMagic 4.0 vector was successfully packaged into lentivirus particles.
CONCLUSIONA lentiviral shRNA expression vector and particles targeting STUB1 gene has been successfully constructed for the further study of the STUB1 gene.
