Detection of RRM1, ERCC1 and BRCA1 gene expression in non-small cell lung cancer tissues and peripheral blood by SYBR real-time fluorescent quantitative PCR.
- Author:
Jian CHEN
1
;
Min-wei LI
;
Guo-bing ZHANG
;
Jun LI
;
Lin-run WANG
Author Information
- Publication Type:Journal Article
- MeSH: Actins; genetics; BRCA1 Protein; blood; genetics; Carcinoma, Non-Small-Cell Lung; blood; metabolism; DNA-Binding Proteins; blood; genetics; Endonucleases; blood; genetics; Female; Humans; Lung Neoplasms; blood; metabolism; Male; Middle Aged; Polymerase Chain Reaction; methods; RNA, Messenger; analysis; blood; Tumor Suppressor Proteins; blood; genetics
- From: Journal of Zhejiang University. Medical sciences 2010;39(6):628-633
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for the detection of RRM1, ERCC1 and BRCA1 gene expression by SYBR real-time fluorescent quantitative PCR in non-small cell lung cancer tissues and peripheral blood.
METHODSThe plasmid standard of RRM1, ERCC1, BRCA1 and β-actin genes was constructed. SYBR real-time PCR was performed, and the standard curve was established. The expressions of RRM1, ERCC1 and BRCA1 mRNA in non-small cell lung cancer tissues and peripheral blood were detected.
RESULTThe standard curve presented linearity. The liquate curves of standard gene were all single apex, indicating that a good specificity was obtained.
CONCLUSIONThe developed SYBR real-time fluorescent quantitative PCR has advantage of convenient operation, low cost, good specificity and high veracity.
