Chiral separation of fluvastatin enantiomers with in vitro cellular method.
- Author:
Bo-xuan QU
1
;
Ye TIAN
;
Lu-shan YU
;
Hui-di JIANG
;
Quan ZHOU
;
Su ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Chromatography, High Pressure Liquid; methods; Fatty Acids, Monounsaturated; isolation & purification; Indoles; isolation & purification; Stereoisomerism
- From: Journal of Zhejiang University. Medical sciences 2011;40(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a chiral separation method for determination of fluvastatin enantiomer with in vitro cellular model.
METHODSThe determination was performed on Chiralpak AD column (4.6 mm × 250 mm); and the phase consisted of hexane-isopropanol-trifluoroacetic acid (90:10:0.1) at a flow rate of 0.5 ml/min with UV detection of 239 nm.
RESULTThe standard curve was linear over the concentration range of 20 μmol/L-300 μmol/L (r² = 0.9993, r² = 0.9997). The recovery for this assay was (99.4 ± 0.8)%, precision for inter-assay and intra-assay was <10 %.
CONCLUSIONThe normal-phase HPLC chiral separation method was accurate and suitable for study on the stereoselectivity of fluvastatin with in vitro cellular model.
