Optimization of alcohol precipitation process for extract of Carthamus tinctorius by multiple guidelines grading method.
- Author:
Jia YUAN
1
;
Ye-rui LI
;
Yong CHEN
;
Long-hu WANG
;
Xue-song LIU
Author Information
- Publication Type:Journal Article
- MeSH: Carthamus tinctorius; chemistry; Chemical Precipitation; Ethanol; chemistry; Plant Extracts; isolation & purification
- From: Journal of Zhejiang University. Medical sciences 2011;40(1):27-32
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the optimal alcohol precipitation parameters for extract of Carthamus tinctorius.
METHODSThe effects of different factors on the transfer rate of hydroxy safflower yellow A (HSYA) was studied via single factor experiments, including the final alcohol concentration of the liquor, the speed of stirring, the initial density of the extract, the temperature and the pH of the liquor. Based on the results of single factor experiments, the final alcohol concentration of the liquor, the speed of stirring, the initial density of the extract and the pH of the liquor were studied by an orthogonal test and a multiple guidelines grading method, and the transfer rate of HSYA, the yield and the purity of extract in the supernatant were used as comprehensive evaluation index.
RESULTThe optimal alcohol precipitation process of Carthamus tinctorius extract was as follows: the final alcohol concentration of the liquor 50%, the speed of stirring 500 r/min, the initial density of the extract 1.15 g/ml and the pH of the liquor 5.0.
CONCLUSIONThe proposed alcohol precipitation process is convenient and steady with high transfer rate of HSYA, high yield and purity of extract in the supernatant.
