Effects of agonist and antagonist of cysteinyl leukotriene receptors on differentiation of rat glioma C6 cells.
- Author:
Bei-Lei CAI
1
;
Xin-Xin WANG
;
Shu-Ying YU
;
Xue-Qin HUANG
;
Xia-Yan ZHANG
;
San-Hua FANG
;
Yun-Bi LU
;
Wei-Ping ZHANG
;
Er-Qing WEI
Author Information
- Publication Type:Journal Article
- MeSH: Acetates; pharmacology; Animals; Cell Differentiation; drug effects; Cell Line, Tumor; Colforsin; pharmacology; Cysteine; Glioma; metabolism; pathology; Leukotriene Antagonists; pharmacology; Leukotriene D4; pharmacology; Leukotrienes; Quinolines; pharmacology; Rats; Receptors, Leukotriene; agonists
- From: Journal of Zhejiang University. Medical sciences 2011;40(2):139-144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of cysteinyl leukotriene (CysLT) receptors in the differentiation of rat glioma C6 cells.
METHODSRat glioma C6 cells were treated with the agonist LTD(4), the CysLT(1) receptor antagonist montelukast and the differentiation inducer forskolin. Cell morphology and GFAP protein expression were determined after treatments.
RESULTForskolin (10 μmol/L) induced morphological changes and GFAP protein expression (cell differentiation) in C6 cells, but LTD(4) (0.1-100 nmol/L) did not induce these changes. Montelukast (1 μmol/L) alone did not affect C6 cell differentiation, while it induced the differentiation when combined with the LTD(4) (100 nmol/L).
CONCLUSIONThe CysLT(2) receptor may modulate the differentiation of rat glioma C6 cells.
