Deficiency of water channel AQP4 aggravates NMDA-induced brain injury in mice.
- Author:
Wen-Zhen SHI
1
;
Chun-Zhen ZHAO
;
Xue-Qin HUANG
;
Wei-Ping ZHANG
;
San-Hua FANG
;
Yun-Bi LU
;
Er-Qing WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aquaporin 4; genetics; physiology; Blood-Brain Barrier; pathology; Brain; drug effects; pathology; Mice; Mice, Knockout; N-Methylaspartate; toxicity
- From: Journal of Zhejiang University. Medical sciences 2011;40(2):145-149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the role of water channel AQP4 in NMDA-induced brain injury in mice.
METHODSIn AQP4 gene knockout (AQP4(-/-)) mice, brain injury was induced by microinjection of NMDA into the cortex. The injured area was determined by toluidine blue staining, degenerated neurons were detected by Fluro-Jade B staining, and increased blood-brain barrier (BBB) permeability was evaluated by IgG immunostaining.
RESULTCompared with wild-type mice, AQP4(-/-) mice exhibited increased cortical lesion area, aggravated neuron degeneration, and increased BBB disruption after NMDA microinjection.
CONCLUSIONAQP4 may play a protective role in NMDA-induced brain injury in mice.
