5-lipoxygenase is involved in rotenone-induced injury in PC12 cells.
- Author:
Xiao-Yan ZHANG
1
;
Li-Hui ZHANG
;
Cheng-Tan LI
;
Wen-Jian CHEN
;
Jian-Bo ZHAO
;
Er-Qing WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arachidonate 5-Lipoxygenase; metabolism; physiology; Cell Survival; drug effects; Hydroxyurea; analogs & derivatives; pharmacology; Lipoxygenase Inhibitors; pharmacology; PC12 Cells; Rats; Rotenone; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2011;40(2):150-155
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine whether 5-lipoxygenase (5-LOX) is involved in rotenone-induced injury in PC12 cells, which is a cell model of Parkinson disease.
METHODSAfter rotenone treatment for various durations, cell viability was determined by colorimetric MTT reduction assay, and 5-LOX translocation was detected by immunocytochemistry. The effect of 5-LOX inhibitor zileuton was also investigated.
RESULTRotenone (0.3-30 μmol/L) induced PC12 cell injury, and zileuton (3-100 μmol/L) attenuated this injury. Rotenone also time-and concentration-dependently induced 5-LOX translocation into the nuclear envelope, and zileuton (1-30 μmo/L) significantly inhibited rotenone-induced 5-LOX translocation.
CONCLUSION5-LOX is involved in rotenone-induced injury in PC12 cells, and 5-LOX inhibitor zileuton can reduce rotenone-induced 5-LOX activation and cell injury.
