Effect of tissue factor in extravascular migration of fibrosarcoma cells.
- Author:
Chun-Shan HAN
1
;
Hua XIN
;
Zhen-Guo HAN
;
Zhong-Li GAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Movement; Cells, Cultured; Endothelial Cells; Fibrosarcoma; metabolism; pathology; Humans; Neoplasm Metastasis; Thromboplastin; metabolism; physiology
- From: Journal of Zhejiang University. Medical sciences 2011;40(2):184-188
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of tissue factor (TF) in extravascular migration of fibrosarcoma cells and hematogenous metastasis.
METHODSThe expression of tissue factor in fibrosarcoma HT1080 cells was analyzed by flow cytometry. The extravascular migration of fibrosarcoma cells was observed in a constructed monolayer vascular endothelial cells and extra-cellular matrix model.
RESULTTissue factor was highly expressed in HT1080 cells. HT1080 migrated and passed through the monolayer vascular endothelial cells to the collagen gel in a time-dependent manner. Anti-TF antibody inhibited extravascular migration of fibrosarcoma cells and the inhibition was concentration-dependent (P<0.05).
CONCLUSIONTissue factor may enhance hematogenous metastasis through extravascular migration of fibrosarcoma cells.
