The comparison of gene expression from human dental pulp cells and periodontal ligament cells.
10.5395/JKACD.2009.34.5.430
- Author:
Hyoun SO
1
;
Sang Hyuk PARK
;
Gi Woon CHOI
Author Information
1. Department of Conservative Dentistry, Division of Dentistry, Graduate of Kyung Hee University, Korea. gwchoi@khu.ac.kr
- Publication Type:Original Article
- Keywords:
cDNA microarray assay;
human dental pulp cells;
human periodontal ligament cells;
RT-PCR;
dentinogenesis;
collagen synthesis
- MeSH:
Collagen;
Dental Pulp;
Dentinogenesis;
Gene Expression;
Humans;
Oligonucleotide Array Sequence Analysis;
Periodontal Ligament
- From:Journal of Korean Academy of Conservative Dentistry
2009;34(5):430-441
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of this study was to characterize functional distinction between human dental pulp cells(PC) and periodontal ligament cells(PDLC) using cDNA microarray assay and to confirm the results of the microarray assay using RT-PCR. 3 genes out of 51 genes which were found to be more expressed(>2 fold) in PC were selected, and 3 genes out of 19 genes which were found to be more expressed(>2 fold) in PDLC were selected for RT-PCR as well. According to this study, the results were as follows: 1. From the microarray assay, 51 genes were more expressed (2 fold) from PC than PDLC. 2. RT-PCR confirmed that ITGA4 and TGF beta2 were more expressed in PC than in PDLC 3. From the microarray assay, 19 genes were more expressed (2 fold) from PDLC than PC. 4. RT-PCR confirmed that LUM, WISP1, and MMP1 were more expressed in PDLC than in PC. From the present study, different expression of the genes between the PC and PDLC were characterized to show the genes which play an important role in dentinogenesis were more expressed from PC than PDLC, while the genes which were related with collagen synthesis were more expressed from PDLC than PC.
