Application of BIOMED-2 primers in analysis of T-cell receptor gamma gene rearrangements in paraffin-embedded tissue specimens of T-cell lymphoma.
- Author:
Yuan TANG
1
;
Wei JIANG
;
Lei LI
;
Hong JI
;
Yun LI
;
Gan-di LI
;
Wei-ping LIU
Author Information
- Publication Type:Journal Article
- MeSH: DNA Primers; DNA, Neoplasm; analysis; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; Humans; Lymphoma, T-Cell; genetics; metabolism; pathology; Paraffin Embedding; Polymerase Chain Reaction; methods; beta-Globins; metabolism
- From: Chinese Journal of Pathology 2009;38(4):253-257
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the practical values of PCR detectable T-cell receptor (TCR) gene rearrangement in paraffin embedded tissue samples in the diagnosis of T-cell malignancies using BIOMED-2 PCR multiplex tubes TCRgamma(A+B).
METHODSTraditional phenol-chloroform method was used to extract DNA from 55 cases of archival paraffin embedded tissues samples of T-cell malignancies and the DNA quality was evaluated by PCR-based amplification of housekeeping gene beta-globin. The selected BIOMED-2 PCR multiplex tubes TCRgamma(A+B) were used to detect TCR gene rearrangement and comparison with the results of universal TCR primers (T(VG)/T(JX)) was performed.
RESULTSPositive detection rates by the BIOMED-2 multiplex tubes TCRgamma(A+B) and the universal primers (T(VG)/T(JX)) were 76.4% and 60.0%, respectively. There were not statistical difference between the methods (P > 0.05).
CONCLUSIONBIOMED-2 multiplex tubes TCRgamma(A+B) is suitable for detection of clonal rearrangements of TCR genes in current archival paraffin embedded tissue samples of T-cell malignancies.
