Overexpression of Tiam1 gene and its relationship with invasive and metastatic ability of nasopharyngeal carcinoma.
- Author:
Xiang-mei ZHANG
1
;
Yi DING
;
Juan-zhi CHEN
;
He JIN
;
Li-na YU
;
Yu-fa LI
;
Yan-qing DING
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion; Cell Line, Tumor; Cell Movement; Gene Expression Regulation, Neoplastic; Guanine Nucleotide Exchange Factors; genetics; metabolism; physiology; Humans; Nasopharyngeal Neoplasms; metabolism; pathology; Neoplasm Invasiveness; Neoplasm Metastasis; Plasmids; RNA, Messenger; metabolism; T-Lymphoma Invasion and Metastasis-inducing Protein 1
- From: Chinese Journal of Pathology 2009;38(4):268-272
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore biological aspects of Tiam1 gene expression in nasopharyngeal carcinoma cells.
METHODSTiam1/C1199HA expression plasmids were transfected into nasopharyngeal carcinoma cells of C666-1 and CNE1 by lipofectamine2000. RT-PCR, real-time PCR and Western blot Analyses were performed to evaluate the expression of Tiam1 mRNA and protein levels, respectively. In vitro cell adhesion, wound healing and matrigel invasion assays were used to study the biological impact of Tiam1 on cell adhesion, mobility and invasion.
RESULTSTiam1 over expression significantly increased the abilities of adhesion, migratory and invasion of C666-1 and CNE1 cells, comparing with that of the control untransfected cells (P < 0.05).
CONCLUSIONTiam1 expression correlates with the invasion and metastasis of nasopharyngeal carcinoma cells.
