Studies on adventitious root induction in vitro and suspension culture of Polygonum multiflorum.
- Author:
He-Ping HUANG
1
;
Shan-Lin GAO
2
;
Jian WANG
3
;
Lu-Qi HUANG
4
;
Peng HUANG
3
Author Information
1. Anhui University of Chinese Medicine, Hefei 230031, China. huangluqi@263.net
2. China Pharmaceutical University, Nanjing 210009, China.
3. Anhui University of Chinese Medicine, Hefei 230031, China.
4. National Resoure Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- MeSH:
Culture Media;
chemistry;
metabolism;
Plant Growth Regulators;
metabolism;
Plant Roots;
growth & development;
metabolism;
Polygonaceae;
growth & development;
metabolism;
Sucrose;
metabolism;
Tissue Culture Techniques;
methods
- From:
China Journal of Chinese Materia Medica
2013;38(22):3857-3860
- CountryChina
- Language:Chinese
-
Abstract:
To achieve sustainable resources use of Polygonum multiflorum, adventitious roots were efficiently induced and cultured by suspension culture. In order to obtain optimal medium for induction adventitious roots from the young stems of P. multiflorum, MS medium was optimized by supplementing with different concentrations of sucrose and plant growth substances. The optimal medium for suspension culture of adventitious roots was determined by orthogonal design. The adventitious roots with suspension culture were subcultured, and the growth curve was also determined. Furthermore, the effective compound in adventitious roots was detected. The result indicated that the optimal medium for efficient induction of adventitious roots was MS medium containing 4% w/v sucrose, supplemented with 2.0 mg x L(-1) NAA, and 0.2 mg x L(-1) 6-BA. The optimal medium for suspension culture of adventitious roots was MS medium containing 3% sucrose, supplemented with 2.0 mg x L(-1) NAA, and 0.2 mg x L(-1) ABT-7.2,3,5,4'-tetrahydroxyl-diphenyl-ethylene-2-O-beta-D-glucoside was detected in adventitious roots, which was effective compound in medicinal material of P. multiflorum. In conclusion, the experiment achieved efficient induction and suspension culture of adventitious roots of P. multiflorum, and laid a foundation for the research on the sustainable use of traditional Chinese medicine resources.
