Bioactivity assay of bupleurum injection for inhibiting PGE2 release in vitro.

Ze-ping Zuo; Zhi-bin Wang; Yang Gao; Yu-dong Guo; Bi-song Wang; Bin SU; Cheng-cheng Song

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Bioactivity assay of bupleurum injection for inhibiting PGE2 release in vitro.

Author: Ze-Ping ZUO ¹ ; Zhi-Bin WANG ² ; Yang GAO ² ; Yu-Dong GUO ² ; Bi-Song WANG ² ; Bin SU ³ ; Cheng-Cheng SONG ³
Author Information

1. Beijing Institute for Drug Control, Beijing 100035, China. zepingzuo@126.com
2. Beijing Institute for Drug Control, Beijing 100035, China.
3. Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
Publication Type:Journal Article
MeSH: Animals; Biological Assay; Bupleurum; chemistry; Cells, Cultured; Dinoprostone; metabolism; Drugs, Chinese Herbal; pharmacology; Female; Hypothalamus; cytology; drug effects; metabolism; Neurons; drug effects; metabolism; Rats; Rats, Sprague-Dawley
From: China Journal of Chinese Materia Medica 2013;38(22):3957-3960
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo establish the in vitro model of PGE2 released by hypothalamic neurocytes under rrIL-1beta in vitro interference, and investigate the correlation of the PGE2 content and the effect of the drug effect concentration in the model under the effect of Bupleurum injection.
METHODHypothalamic neurocytes were cultured in vitro, and added with rrIL-1beta (40 microg x L(-1)) stimulation. Cell sap was collected at different time points. ELISA was adopted to determine the content of PGE2 in cell sap collected at different time points. Hypothalamic neurocytes were cultured in vitro, added with rrIL-1beta (40 microg x L(-1)) stimulation and then different concentrations of Bupleurum injection. The changes in the content of PGE2 in cell supernatant were detected by ELISA. An analysis was made on the linear relationship between the sample concentration and the inhibition rate of PGE2.
RESULTThe rrIL-1 cells could stimulate in vitro cultured hypothalamic neurocytes to release PGE2 and reach the peak at 10 h. Bupleurum injection could significantly interfere the release of PGE2 in the in vitro model (P < 0.01, P < 0.05), with a certain linear relationship between the interference effect and the effect concentration of Bupleurum injection (r = 0.911, P < 0.01).
CONCLUSIONThe rrIL-1 cells could stimulate in vitro cultured hypothalamic neurocytes to release PGE2, with a good correlation between the inhibition and generation effects of PGE2 and the drug concentration.