Chemical structural features and anti-complementary activity of polysaccharide HPS1-D from Hedysarum polybotrys.
- Author:
Tao YANG
;
Long GUO
;
Can LI
;
Ying-Lai YANG
;
Shi-Lan FENG
- Publication Type:Journal Article
- MeSH:
Animals;
Drugs, Chinese Herbal;
chemistry;
pharmacology;
Hemolysis;
drug effects;
Mice;
Plant Extracts;
chemistry;
pharmacology;
Polysaccharides;
chemistry;
pharmacology
- From:
China Journal of Chinese Materia Medica
2014;39(1):89-93
- CountryChina
- Language:Chinese
-
Abstract:
HPS1-D, an active polysaccharide,was isolated and purified from Hedysarum polybotrys. HPS1-D was obtained after treated with Savage method and H2O2, and purified with DEAE-cellulose 52 and Sephadex G-100 gel filtration chromatography. Then physicochemical property analysis, GC, methylation, partial acid hydrolysis, and NMR method were used to study chemical structural of HPS1-D. The conformation was primarily analyzed with GPC-MALLS method and Congo red reaction. The anti-complementary activity of HPS1-D was evaluated with the hemolysis assay. HPS1-D was a heteropolysaccharide and consisted of D-glucose, L-arabinose, (7.2:1.3). HPS1-D proved to be a neutral sugar, with 1, 4-and 1, 4, 6-alpha-D-glucopyranosyl residues in backbone ,and 1, 5-and 1, 3, 5-alpha-L-arabinofuranosyl residues in branches. HPS1-D has a random coil state conformation with monodisperse mass distribution in 0.9% NaCl solution. And HPS1-D had triple-helix conformation in concentrate of NaOH solution. Anti-complementary activity of HPS1-D was closed to its positive control heparin.
