Analysis of monosaccharide compositions in polysaccharides from exopleura of Ginkgo biloba.
- Author:
Lei-Lei MAO
;
Ying CHEN
;
Bi-Yuan HU
;
Ai-Hua XU
- Publication Type:Journal Article
- MeSH:
Ginkgo biloba;
chemistry;
Hydrolysis;
Monosaccharides;
analysis;
Plant Components, Aerial;
chemistry;
Polysaccharides;
chemistry
- From:
China Journal of Chinese Materia Medica
2014;39(2):262-266
- CountryChina
- Language:Chinese
-
Abstract:
A high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5 -pyrazolone (PMP) has been established for determination of 6 kinds of monosaccharides simultaneously. A special Agilent HC-C18 column (4. 6 mm x 250 mm, 5 microm), optimized for the separation of PMP derivatives, was used at ambient temperature of 40 degrees C. The PMP derivatives elution was performed with a mixture of 0.1 mol x L(-1) phosphate buffer (pH 6. 8) and acetonitrile in a ratio of 84: 16 at a flow rate of 1 mL x min(-1), and UV absorbance of the effluent was monitored at 245 nm. The results showed that the polysaccharides from exopleura of Ginkgo biloba were acidic heteropolysaccharides mainly containing mannose, rhamnose, D-galacturonic acid, glucose, galactose, arabinose, with the molar ratio of 0.032: 0.14: 0.296: 0.403:0.106: 0.046.
