The highly expressed secreted phosphoprotein 1 gene in prostate cancer metastasis: a microarray-based bioinformatic analysis.
- Author:
Tie-qiu LI
1
;
Yi-li TENG
;
Ya-guang ZOU
;
Yu YANG
;
Qi LI
;
Xiang-ming MAO
Author Information
- Publication Type:Journal Article
- MeSH: Computational Biology; Data Mining; Down-Regulation; Humans; Male; Microarray Analysis; Osteopontin; chemistry; genetics; secretion; Phosphatidylinositol 3-Kinases; metabolism; Prostatic Neoplasms; genetics; metabolism; pathology; Signal Transduction; Toll-Like Receptors; metabolism
- From: National Journal of Andrology 2014;20(11):984-990
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the composition, function, and regulatory mechanisms of the secreted phosphoprotein 1 (SPP1) gene in metastatic prostate cancer.
METHODSWe obtained the data about the whole genomic expression profiles on prostate cancer metastasis from the GEO database, and performed data-mining and bioinformatic analysis using BRB-Array Tools and such softwares as Protparam, MotifScan, SignalP 4.0, TMHMM, NetPhos2.0, PredictProtein, GO, KEGG, and STRING.
RESULTSTotally, 73 co-expressed differential genes in prostate cancer metastasis were identified, 21 up-regulated and 52 down-regulated (P <0.01). Bioinformatic analysis indicated that the highly expressed SPP1 gene encoded 314 amino acids and contained 2 N-glycosylation sites, 8 casein kinase II phosphorylation sites and 3 protein kinase C phosphorylation sites, playing essential roles in extracellular matrix (ECM) binding, ossification, osteoblast differentiation, cell adhesion, PI3K-Akt signaling pathway, focal adhesion, Toll-like receptor signaling pathway, and ECM-receptor interaction.
CONCLUSIONThe bioinformatic method showed a high efficiency in analyzing microarray data and revealing internal biological information. SPP1 may play an important role in prostate cancer metastasis and become a novel biomarker for the diagnosis of prostate cancer metastasis and a new target for its treatment.
