Protective effect of tadalafil against ischemia-reperfusion injury in rats.
- Author:
Zhi-gang WU
;
Guang-bing WANG
;
Yun-bei XIAO
;
Tong-ke CHEN
;
Jian CAI
;
Cheng-di LI
- Publication Type:Journal Article
- MeSH: Animals; Biomarkers; metabolism; Carbolines; administration & dosage; pharmacology; Dose-Response Relationship, Drug; Male; Malondialdehyde; metabolism; Phosphodiesterase 5 Inhibitors; pharmacology; Random Allocation; Rats; Rats, Sprague-Dawley; Reperfusion Injury; prevention & control; Seminiferous Tubules; pathology; Spermatic Cord Torsion; complications; Superoxide Dismutase; metabolism; Tadalafil; Testis; blood supply; metabolism; pathology; Time Factors
- From: National Journal of Andrology 2015;21(3):214-218
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of phosphodiesterase type 5 inhibitors (tadalafil) on the testis following testicular ischemia-reperfusion injury in rats.
METHODSEighty-four healthy adult male SD rats were randomly and equally divided into groups A (sham operation), B (testicular torsion + low-dose tadalafil), C (testicular torsion + high-dose tadalafil), and D (testicular torsion + placebo). Models were established in the latter three groups by 7200 torsion of the right testis for 2 hours. The animals in groups A and B were treated by gavage with tadalafil at the dose of 0. 5 mg per kg per day, those in group C at 2 mg per kg per day, and those in group D with saline at the same dose. After 3, 7, and 14 days of treatment, the torsioned testes were harvested for evaluation of the superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in the testis tissue. The pathological changes in the testis were observed under the light microscope.
RESULTSAt 3, 7, and 14 days, the SOD activity was (254.46 +/- 7.43), (278.49 +/- 8.33), and (317.99 +/- 3.31) nU/mg prot in group B, and (277.12 +/- 8.80), (309.40 +/- 2.14), and (320.39 +/- 4.72) nU/mg prot in group C, all obviously higher than in D ([223.21 +/- 4.65], [231.45 +/- 4.16] and [248.28 +/- 5.74] nU/mg prot), while the MDA content was lower in the former two groups than in the latter. At 3 and 7 days, the SOD activity was significantly higher and the MDA level significantly lower in group C than in B (both P < 0.01) , while at 14 days, neither showed any remarkable differences between the two groups (P > 0.05). No obvious histopathological change was observed in the testis tissue of group A. At 3 and 7 days, pathological examination of the testis tissue revealed significant differences in the number of seminiferous epithelial layers, testicular histological score, and seminiferous tubule diameter in group B (P < 0.01), but the three indexes at 14 days in group B and at 7 days in group C exhibited no remarkable differences from those at 14 days in group A.
CONCLUSIONTadalafil can alleviate testicular ischemia-reperfusion injury following testis torsion/detorsion in a time- and dose-dependent manner.