Effects of gastrin on rat intestinal epithelial 1,25(OH)2D3-membrane associated rapid response steroid binding protein.
- Author:
Fenfen LIANG
1
;
Cuiping LIU
;
Lixuan LI
;
Yu GUO
;
Lan BAI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcitriol; metabolism; Epithelial Cells; drug effects; metabolism; Gastrins; pharmacology; Intestines; cytology; drug effects; Male; Protein Disulfide-Isomerases; metabolism; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2013;33(7):990-993
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of gastrin on the expression of 1,25(OH)2D3-membrane associated rapid response steroid (1,25D3-MARRS) binding protein in rat intestinal epithelium.
METHODSSD rats received intraperitoneal injections of gastrin, omeprazole or physiological saline. The protein expression of 1,25D3-MARRS binding protein in SD rat intestinal was determined with Western blotting and immunohistochemistry, and its mRNA levels determined by RT-PCR. The serum calcium and phosphate levels in the rats were also detected.
RESULTSImmunohistochemistry showed that 1,25D3-MARRS binding protein was expressed mainly in the nuclei, cytoplasm and membrane of the intestinal epithelial cells. Both the protein and mRNA expression levels of 1,25D3-MARRS binding protein were up-regulated after treatments with gastrin and omeprazole (P<0.05), but the serum calcium and phosphate concentrations showed no obvious increase.
CONCLUSION1,25D3-MARRS binding protein, which is widely expressed with versatile functionalities, is regulated by gastrin and shows high potentials in the study of gastrointestinal diseases.
