La protein expression in cervical cancer tissues and its clinical significance.
- Author:
Kunlun CHEN
1
;
Yi WU
;
Mu LI
;
Lan LI
;
Yane GAO
;
Qing GAO
Author Information
- Publication Type:Journal Article
- MeSH: Autoantigens; metabolism; Cell Cycle Checkpoints; Cyclin D1; metabolism; Female; HeLa Cells; Humans; RNA Interference; RNA-Binding Proteins; metabolism; Ribonucleoproteins; metabolism; Uterine Cervical Neoplasms; metabolism; pathology
- From: Journal of Southern Medical University 2013;33(7):999-1003
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of La protein in cervical cancer tissues and explore its role in the occurrence and progression of cervical cancer.
METHODSThe expression of La protein in cervical cancer and normal cervical tissues was detected by immunohistochemical staining. RNA interference technology was used to silence La protein expression in HeLa cells and the changes in cell proliferation, tumor sphere formation and cell cycles were investigated.
RESULTSThe expression of La protein was significantly higher in cervical cancer tissues than in normal cervical tissues (61% vs 9%, P<0.05). Silencing La protein expression in HeLa cells caused significantly reduced the cell proliferation and lowered the tumor sphere formation rate from the control level of (17.1=1.92)% to (6.3=0.45)% (P<0.05), resulting also in G0/G1 cell cycle arrest and reduced cyclin D1 protein expression.
CONCLUSIONThe RNA binding protein La can promote the development of cervical cancer and may play a critical role in the carcinogenesis and progression of cervical cancer.
