Origin and the recombinant model of H7N9 virus prevailing in China.
- Author:
Bao ZHANG
1
;
Keyong HUANG
;
Jinsong GUO
;
Xianbo WU
;
Ling LI
;
Li ZHU
;
Chengsong WAN
;
Wei ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: China; Genome, Viral; Genotype; Humans; Influenza A Virus, H7N9 Subtype; classification; genetics; isolation & purification; Influenza, Human; virology; Phylogeny; Reassortant Viruses; classification; genetics; isolation & purification; Sequence Analysis, RNA; Viral Proteins; genetics
- From: Journal of Southern Medical University 2013;33(7):1017-1021
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the origin and the recombinant model of H7N9 virus prevailing in China by sequence analysis.
METHODSThe sequences of H7N9 virus were collected and analyzed with the software BLAST and MEGA 5.0. The phylogenetic trees were constructed after multiple sequences alignment. The homologous sequences of H7N9 segments were determined and the model was inferred according to the origin of H7N9 segments.
RESULTSThe most relevant sequences of HA, NA, NS and PB2 segments were located at one branch of the phylogenetic tree, while the closest relevant sequences of PB1, PA, NP and MP contained two H9N2 virus origins. According to the analysis of sequence origin, H7N9 viruses might be divided into 5 genotypes: namely A, B, A/Shanghai/1/2013-H7N9, A/Pigeon/Shanghai/S1069-H7N9 and A/Zhejiang/HZ1/2013-H7N9, and the genotype A consisted of A1 and A2 subtypes.
CONCLUSIONThe prevailing H7N9 virus might be derived from 5 different viruses after 4 times of recombination, which resulted in the two major types of A and B. The subtypes of A1 and A2 were two different derivatives from one reassortant. The A/Pigeon/Shanghai/S1069-H7N9 strain might be the recombinant of type A H7N9 virus with a local H9N2 virus during the H7N9 epidemics. The A/Zhejiang/HZ1/2013-H7N9 strain could be the re-arrangement of subtype A2 with type B H7N9 virus.
