Application of pyrosequencing in detection of common pathogens in sepsis.
- Author:
Ziyou HU
1
;
Hui HAN
;
Yong ZENG
;
Bingyi WU
Author Information
- Publication Type:Journal Article
- MeSH: Bacteria; classification; isolation & purification; DNA Primers; DNA, Bacterial; genetics; Microbiological Techniques; Polymerase Chain Reaction; RNA, Ribosomal, 16S; Sepsis; microbiology
- From: Journal of Southern Medical University 2013;33(7):1053-1056
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo apply pyrosequencing technique in the detection of the common pathogens in sepsis.
METHODSThe primers for amplification and sequencing in pyrosequencing were designed according to alignment of the bacterial 16S rRNA sequence. Bacterial genomic DNA was extracted for pyrosequencing, and the pathogen species were determined according to the sequencing data obtained.
RESULTSPyrosequencing effectively yielded the sequencing data of the 28 bp sequences of the pathogens and clearly distinguished the pathogen species of Streptococcus pyogenes, Streptococcus pneumonia, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Neisseria meningitides, and Salmonella, but failed to distinguish Staphylococcus epidermidis from Staphylococcus aureus.
CONCLUSIONPyrosequencing technique can effectively distinguish the common pathogens in sepsis at the species level.
