MicroRNA-218 expression and its role in hepatocellular carcinoma.
- Author:
Chao LI
1
;
Kangsheng TU
;
Xin ZHENG
;
Jing ZHANG
;
Hang TUO
;
Jie GAO
;
Yingmin YAO
;
Qingguang LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Apoptosis; Carcinoma, Hepatocellular; genetics; metabolism; pathology; Cell Proliferation; Cyclin-Dependent Kinase 6; metabolism; Female; Hep G2 Cells; Humans; Liver Neoplasms; genetics; metabolism; pathology; Male; MicroRNAs; genetics; metabolism; Middle Aged; Polycomb Repressive Complex 1; metabolism
- From: Journal of Southern Medical University 2013;33(8):1127-1131
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of microRNA-218 (miR-218) and its role in hepatocellular carcinoma (HCC).
METHODSForty-six pairs of fresh surgical specimens of HCC and adjacent tissues were examined for miR-218 expression using qRT-PCR. A miR-218 mimic was transfected into HepG2 cells, and the cell viability and apoptosis were analyzed by MTT assay and flow cytometry, and the potential targets of miR-218 were detected by qRT-PCR and Western blotting.
RESULTSThe expressions of miR-218 in HCC tissues were significantly down-regulated compared to those in the adjacent tissues (P<0.05). Down-regulation of miR-218 was found to correlate significantly with the tumor size (>5 cm) and an advanced TNM stage (III+IV) (P<0.05). Ectopic expression of miR-218 in HepG2 cells resulted in suppressed cell proliferation and enhanced cell apoptosis as well as the down-regulation of Bmi-1 and CDK6 mRNA and protein expressions (P<0.05).
CONCLUSIONThe low-expression of miR-218 is correlated with malignant clinicopathological characteristics of HCC, and miR-218 may inhibit cell proliferation and promote cell apoptosis by down-regulating Bmi-1 and CDK6 in HCC.
