Analysis of capsaicin in rat plasma using liquid chromatography-tandem mass spectrometry.
- Author:
Lian DUAN
1
;
Yuan YAN
;
Yuan LIU
;
Sijia LIU
;
Guofeng LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Capsaicin; blood; Chromatography, High Pressure Liquid; Plasma; chemistry; Rats; Tandem Mass Spectrometry
- From: Journal of Southern Medical University 2013;33(8):1165-1168
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a simple high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS) system for determination of capsaicin in rat plasma.
METHODSRat plasma (100 µl) was extracted with mixed solvent (ethyl acetate:acetone 85:15) after addition of 50 ng/ml internal standard, and analyzed by Agilent 6460 Triple Quadrupole LC/MS system. The detection was performed with multiple reactions monitoring using electrospray ionization. The precursor/product ion transitions were monitored at m/z 306→137 (+Ion mode) for capsaicin. Verapamil was used as the internal standard at m/z455→165.
RESULTSThe analysis time was only 1.5 min in the positive mode; the calibration curve was linear in the concentration range of 1.85-370 ng/ml. The lowest limit of quantification reached 1.85 ng/ml. The extraction recoveries were 77.34%, 70.64% and 78.02% for the three quality control concentration levels (3.7, 37, and 370 ng/ml), respectively.
CONCLUSIONThe LC-MS/MS system is simple, accurate, reliable and time-saving for determination of trace amount of capsaicin in plasma.