Recombinant human bone morphogenetic protein-2 down-regulates LASP1 and up-regulates ferritin during osteogenic differentiation of beagle dog bone marrow mesenchymal stem cells.
- Author:
Jijie HU
1
;
Yawei LIU
;
Minyi HE
;
Bin YU
;
Gang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; metabolism; Animals; Bone Marrow Cells; cytology; drug effects; Bone Morphogenetic Protein 2; pharmacology; Cell Differentiation; drug effects; Cells, Cultured; Cytoskeletal Proteins; metabolism; Dogs; Down-Regulation; Ferritins; metabolism; LIM Domain Proteins; metabolism; Mesenchymal Stromal Cells; cytology; drug effects; Osteogenesis; drug effects; Proteomics; Recombinant Proteins; pharmacology; Transforming Growth Factor beta; pharmacology; Up-Regulation
- From: Journal of Southern Medical University 2013;33(8):1207-1212
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the proteins involved in osteogenic differentiation of beagle dog bone marrow mesenchymal stem cells (BMSCs) and explore its possible regulation mechanism of osteogenic differentiation of BMSCs.
METHODSCultured beagle dog BMSCs were induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) for 7 days. The differentially expressed proteins between cells with osteogenic differentiation and control cells were identified by proteomics analysis based on two-dimensional gel electrophoresis. Q-PCR and Western blotting were used to verify the interested protein of LASP1, ferritin light chain and heavy chain.
RESULTSOsteogenic differentiation was induced successfully in the BMSCs. Twenty differentially expressed proteins were identified by proteomic analysis, including 9 down-regulated and 11 up-regulated ones. Q-PCR and Western blotting demonstrated a significant reduction of LASP1 expression and significant up-regulation of ferritin in the BMSCs after a 7-day induction with rhBMP-2.
CONCLUSIONLASP1, which plays an important role in the regulation of the activity of the cytoskeleton, and ferritin, an important molecule in cellular iron homeostasis, can be critical in the osteogenic differentiation of beagle dog BMSCs induced by rhBMP-2.
