Expression of angiopoietin-2 and vascular endothelial growth factor in human colon cancer.
- Author:
Ruihua WANG
1
;
Jianguo XIE
;
Hua CHEN
;
Tingting REN
;
Yicui ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Angiopoietin-2; blood; metabolism; Case-Control Studies; Colorectal Neoplasms; blood supply; metabolism; pathology; Female; Gene Expression Regulation, Neoplastic; Humans; Male; Middle Aged; Neovascularization, Pathologic; Vascular Endothelial Growth Factor A; blood; metabolism
- From: Journal of Southern Medical University 2013;33(8):1236-1239
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association of the expressions of angiopoietin-2 (Ang-2), vascular endothelial growth factor (VEGF) in colorectal cancer tissues with Dukes' clinicopathological features.
METHODSAng-2 and Tie-2 mRNA expressions were detected in colorectal cancer tissues, adjacent tissues, and normal tissues by real time-PCR. Quantikine immunoassays were used to measure the protein expressions of Ang-2 and VEGF in the tissues and serum samples.
RESULTSAng-2 and Tie-2 levels were significantly higher in the serum of the patients than in the normal tissues (P<0.05), and their expressions were strongly correlated (r=0.879, P=0.000). Tumor tissue Ang-2 and VEGF levels were significantly higher than their levels in the adjacent and normal tissues (P<0.05). In colorectal cancer patients, the peripheral blood level of Ang-2 was significantly higher than that in healthy control subjects, and comparable with that in mesenteric blood (P>0.05). In Dukes' stage C and D patients, serum Ang-2 and VEGF levels were significantly higher than those in patients in Dukes' stage A and B (P<0.05).
CONCLUSIONAng-2 and VEGF over expressions may play an important role in the occurrence and progression of colorectal cancer.
