Effect of the C-terminal truncated human apoptosis-inducing factor δ1-480 on biological behaviors of MCF-7 cells.
- Author:
Lijuan WANG
1
;
Huawei XIAO
;
E BAI
;
Xia ZHOU
;
Meng LI
;
Guihua JIN
;
Qing ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Apoptosis Inducing Factor; genetics; metabolism; Breast Neoplasms; metabolism; pathology; Cell Proliferation; Cytochromes c; genetics; metabolism; Female; Humans; MCF-7 Cells; Membrane Potential, Mitochondrial; Mitochondria; metabolism
- From: Journal of Southern Medical University 2013;33(9):1285-1288
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression of the C-terminal truncated human apoptosis-inducing factor (AIF) and its biological effect on MCF-7 cells.
METHODSPcDNA3.0-FDT-AIFδ1-480 was transfected into human breast carcinoma MCF-7 cells with lipofectamine. The expression of the truncated AIF gene was detected by Western blotting, and its effects on the biological behaviors of MCF-7 cells and on the expression of cytochrome c (cytC) were evaluated using flow cytometry, MTT assay, colony-forming assay, and mitochondrial membrane potential measurement.
RESULTSPcDNA3.0-FDT-AIFδ1-480 enhanced AIF expression in MCF-7 cells, obviously inhibited the cell proliferation, and significantly reduced the mitochondrial membrane potentials (P<0.05). Transfection of the cells with PcDNA3.0-FDT-AIFδ1-480 promoted the expression of cytC and resulted in significantly increased apoptosis of MCF-7 cells (P<0.05).
CONCLUSIONThe expression of C-terminal truncated human AIF gene can induce apoptosis of human MCF-7 cells by promoting cytC release from mitochondria.
