Expression of Engrailed-2 and β-catenin in bladder urothelial carcinoma and their significance.
- Author:
Yunfei LI
1
;
Haitao LIU
;
Xinghua DU
;
Caiyong LAI
;
Zexuan SU
;
Shuangquan GAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Carcinoma; metabolism; pathology; Female; Homeodomain Proteins; metabolism; Humans; Male; Middle Aged; Nerve Tissue Proteins; metabolism; Urinary Bladder Neoplasms; metabolism; pathology; beta Catenin; metabolism
- From: Journal of Southern Medical University 2013;33(9):1372-1376
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expressions of Engrailed-2 (EN2) and β-catenin in bladder urothelial carcinoma and explore their significance.
METHODSSixty bladder urothelial carcinoma samples of different grades and stages and 10 normal bladder mucosal tissues were examined for expressions of EN2 and β-catenin proteins and mRNA using immunochemistry, Western blotting and RT-PCR. RESULTS Compared to normal bladder mucosa, bladder urothelial carcinoma tissues showed significantly increased expressions of EN2 and β-catenin proteins (P<0.05), and the high-grade carcinoma tissues exhibited significantly stronger expressions than the low-grade ones (P<0.05); the expressions of the proteins increased also significantly with advanced pathological stages of bladder urothelial carcinoma (P<0.05). The expressions of EN2 and β-catenin mRNAs showed a consistent pattern of changes with their protein expressions.
CONCLUSIONThe expressions of EN2 and β-catenin are significantly increased in bladder urothelial carcinoma. EN2 may contribute to the development and progression of bladder urothelial carcinoma by activating Wnt/β-catenin signal pathway.
