Optimization of acoustic parameters of microbubble-enhanced ultrasound sonoporation for augmenting tumor cell permeability in vivo.
- Author:
Lihong LUO
1
;
Fenglin WU
;
Baoping WANG
;
Tingting XU
;
Jie HU
;
Yang SUI
Author Information
- Publication Type:Journal Article
- MeSH: Acoustics; Animals; Cell Line, Tumor; Cell Membrane Permeability; Microbubbles; Neoplasms; therapy; Rabbits; Ultrasonic Therapy; Ultrasonics
- From: Journal of Southern Medical University 2013;33(9):1377-1381
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the appropriate acoustic parameters for augmenting the permeability of tumor cells using microbubble-enhanced ultrasound.
METHODSNinety intramuscular VX2 tumors grown in New Zealand white rabbits were randomly divided into 4 parts by 4 factors, namely sound intensity, pulse width, insonation time and microbubble dose, each at different levels. Therapeutic ultrasound was delivered directly to the tumor surface during intravenous infusion of microbubbles. The tumor was excised after the treatment and sliced to investigate the change in tumor cell permeability using lanthanum nitrate electron microscopy. The appropriate acoustic parameters of the 4 factors were investigated.
RESULTSSonoporation induced by microbubble-enhanced ultrasound augmented the permeability of the tumor cells, and the appropriate sound intensity, pulse width, insonation time and microbubble dose were 0.43 W/cm(2), 2.42 ms, 10 min, and 0.50 ml/kg, respectively.
CONCLUSIONSonoporation induced by microbubble-enhanced ultrasound can induce pores in tumor cell membrane, which may potential increase the efficacy of tumor chemotherapy.
