Diammonium glycyrrhizinate inhibits airway smooth muscle proliferation in a murine model of chronic asthma.
- Author:
Min XIAO
1
;
Tao ZHU
;
Tao WANG
;
Fuqiang WEN
Author Information
- Publication Type:Journal Article
- MeSH: Actins; genetics; metabolism; Airway Remodeling; drug effects; Animals; Anti-Inflammatory Agents; pharmacology; Asthma; metabolism; pathology; Cell Proliferation; drug effects; Collagen; metabolism; Glycyrrhizic Acid; pharmacology; Lung; metabolism; pathology; Male; Mice; Mice, Inbred BALB C; Muscle, Smooth; pathology; Ovalbumin; pharmacology; PPAR gamma; genetics; metabolism; RNA, Messenger; metabolism; Random Allocation; Up-Regulation
- From: Journal of Southern Medical University 2013;33(10):1416-1420
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the therapeutic value and possible mechanism of diammonium glycyrrhizinate (DG) in treatment of airway remodeling in a murine model of chronic asthma.
METHODSThirty male BALB/C mice were randomly divided into control group, OVA+DG group and OVA group (n=10). HE staining was used to observe the pathological changes, and Masson's staining was used to detect and measure collagen deposition. Alpha-SMA and PPARγ mRNA expressions were analyzed by RT-PCR, and the protein expressions of α-SMA and PPARγ were measured by Western blotting.
RESULTSAfter 75 days of OVA sensitization and challenge, obvious pathological changes occurred in the lung tissues, which was more severe in OVA group than in OVA+DG group. Collagen deposition was significantly increased after OVA stimulation, but was obviously milder in OVA+DG group than in OVA group. OVA-induced up-regulation of α-SMA was notably attenuated by DG injection. The expression of PPARγ was markedly down-regulated after OVA stimulation but was substantially enhanced after DG intervention.
CONCLUSIONDG can inhibit airway smooth muscle proliferation possibly through up-regulation of PPARγ in a murine model of chronic asthma.
