Neurogenesis in the dentate gyrus of the hippocampus in a rat model of intracerebral hemorrhage.
- Author:
Xi XU
1
;
Jianyun ZHENG
;
Junfeng ZHANG
;
Xinlin CHNE
;
PengBo YANG
;
Jianxin LIU
;
Yong LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Nuclear; metabolism; Bromodeoxyuridine; metabolism; Cerebral Hemorrhage; metabolism; pathology; physiopathology; Dentate Gyrus; metabolism; physiopathology; Hippocampus; metabolism; physiopathology; Microtubule-Associated Proteins; metabolism; Nerve Tissue Proteins; metabolism; Neurogenesis; physiology; Neurons; metabolism; physiology; Neuropeptides; metabolism; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2013;33(10):1437-1441
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether intracerebral hemorrhage (ICH) can promote neurogenesis in the dentate gyrus (DG) of rat hippocampus.
METHODSWestern blot analysis, immunohistochemical staining, and immunofluorescent double labeling combined with confocal microscope were used to detect neurogenesis in the DG of the hippocampus in rats after ICH.
RESULTSThe expression of DCX protein in the ipsilateral DG of the hippocampus was enhanced in the rats 1 day after ICH (0.202∓0.062) as compared with that in normal rats (0.127∓0.088), reaching the peak level at 14 days (0.771∓0.108, P<0.01) and beginning to decrease at 28 days (0.582∓0.121, P<0.01). Meanwhile, DCX-positive cells and BrdU-positive cells, and DCX/BrdU double-labeled cells were detected in the DG of the hippocampus. Compared with those in the control group, BrdU/NeuN double-labeled cells were markedly increased in the granular cell layer of the DG at 28 days after ICH (1.808∓1.020 vs 5.654∓1.671, P<0.01).
CONCLUSIONICH can promote neurogenesis in the DG of rat hippocampus.
