Effects of hypoxic-preconditioning on anoxic-tolerance and Jun expression in cultured rat hippocampal neurons.
- Author:
Ai-Shi DING
1
;
Fu-Zhuang WANG
;
Shun YU
;
Li-Ying WU
;
Tong ZHAO
;
Ming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Hypoxia; Cells, Cultured; Genes, jun; Hippocampus; metabolism; Neurons; metabolism; Oxygen; metabolism; Rats; Rats, Wistar
- From: Chinese Journal of Applied Physiology 2002;18(1):26-29
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effects of hypoxic preconditioning on anoxic tolerance and Jun expression in cultured rat hippocampal neurons after anoxia/reoxygenation.
METHODS12 day cultured hippocampal neurons in control and hypoxic preconditioning group were exposed to anoxic environment (0.90L/L N2 + 0.10 L/L CO2) for 4 h, and then reoxygenated for either 24 h or 72 h. The neurons were immunocytochemically stained using the antiserum against Jun. The number of survival neurons and the percentage of Jun expressing neurons were investigated.
RESULTSThe percentage of Jun expressing neurons induced by anoxia in hypoxic-preconditioning group was significantly less than that in control group. The number of survival neurons was more in the hypoxic-preconditioning group than that in control group after anoxic reoxygenation.
CONCLUSIONHypoxic-preconditioning can induce the development of anoxic-tolerance in cultured hippocampal neurons. The decrease in Jun expressing neurons in hippocampus may be an adaptive reaction to acute anoxia.
