Studies of protective effect of SOD on damage of human RBCs stored at 4 degrees C.
- Author:
En-Pu MA
1
;
Xiu-Zhen LIU
;
Ying HAN
;
Su-Ping REN
;
An LIN
;
Peng JIN
;
Xing LÜ
;
Zu-Ze WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Preservation; methods; Erythrocytes; cytology; metabolism; Humans; Rabbits; Refrigeration; Superoxide Dismutase; pharmacology
- From: Chinese Journal of Applied Physiology 2002;18(1):80-83
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the mechanism of protective effect of SOD (superoxide dismutase) on damage of RBCs stored at 4 degrees C, the studies of erythrocyte glucose and energy metabolism were performed.
METHODSwhole blood collected from healthy donors and stored at 4 degrees C in ACD, GMA and SOD solutions. Before and post storage, some parameters were assayed. Standard methods were used for the in vitro tests. The 24-hour in vivo recoveries were measured by FTTC (Fluorescein 5-isothiocyanate) from SIGMA Company.
RESULTSAll parameters of red blood cell glucolysis rate without oxygen condition, ATP, PK (pyruvic kinase) and 24 h recoveries level were 86.2%, 56.4%, 64.3% and 86.2% of normal respectively stored in SOD solution at 4 degrees C for 75 days, distinctly more than in ACD and GMA groups at 75 days stored. The 24 h recovery at 75d in group SOD was near the recovery at 42d in group GMA.
CONCLUSIONWhole blood in SOD solution can be stored satisfactorily for 75 days at 4 degrees C, and furnished theoretical evidence for RBCs survival.
