Activation of PACAP receptor could protect cultured hippocampal neurons against beta amyloid peptide induced neurotoxicity.
- Author:
Lan-Run GUI
1
;
Wen-Bin LI
;
Bing-Lie ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Peptides; toxicity; Animals; Apoptosis; drug effects; Cells, Cultured; Hippocampus; cytology; Neurons; drug effects; metabolism; Rats; Rats, Sprague-Dawley; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; metabolism
- From: Chinese Journal of Applied Physiology 2002;18(2):145-148
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the protective role of pituitary adenylate cyclase activating polypeptide (PACAP) on hippocampal neuronal apoptosis induced by beta amyloid peptide in the culture.
METHODSHippocampal neurons were isolated from 1d old SD rat and neuronal survival and apoptosis were measured by MTT assay and DNA ladder.
RESULTS25 micromol/L Abeta could induce neuron apoptosis while co-treatment with PACAP could increase the survival of hippocampal neurons. The antagonist of PACAP receptor, P6-27, could reverse the effect of PACAP.
CONCLUSIONPACAP could protects cultured neurons from the neurotoxicity of Abeta through the activation of PACAP receptor and may have a bright use in treatment of neurodegenerative disease.
