Effect and mechanism of interleukin-2 on the rhythm of isolated rat heart and neonatal myocytes.
- Author:
Lin-Lin WANG
1
;
Ying-Ying CHEN
;
Qiang XIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cells, Cultured; Heart; drug effects; Heart Rate; drug effects; In Vitro Techniques; Interleukin-2; pharmacology; Myocytes, Cardiac; drug effects; physiology; Propranolol; pharmacology; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2002;18(2):149-151
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore the effect and mechanism of interleukin-2 (IL-2) on the rhythm of isolated rat heart and neonatal myocytes.
METHODSCultured neonatal rat cardiomyocytes and isolated perfused rat heart were used.
RESULTS(1) IL-2 (2.5-200 u/ml) reduced the spontaneous beating rate of cultured neonatal rat cardiomyocytes in a dose-dependent manner. (2) IL-2 at 50 u/ml increased both the heart rate and the number of premature ventricular excitation in the isolated heart. (3) Pretreatment with propranolol abolished the effect of 50 u/ml IL-2 on the isolated heart. (4) Heat inactivated IL-2 had no significant effect on the cultured cardiomyocytes and isolated hearts.
CONCLUSIONIL-2 inhibited the auto rhythmic of cultured cardiomyocyte directly while the positive chronotropic and arrhythmogenic effects of IL-2 in the isolated rat heart may be mediated by endogenous catecholamine.
