Effect of Puerarin on K+ channel of isolated ventricular myocyte in guinea pig.

Author: Wei-Na MIAO ¹ ; Ying-Jun SHEN ; Xiao-Rong ZENG
Author Information

1. Department of Physiology, Pharmacology Cheng Du University of Traditional Chinese Medicine, Chengdu 610075, China.
Publication Type:Journal Article
MeSH: Animals; Guinea Pigs; Heart Ventricles; cytology; Isoflavones; pharmacology; Myocytes, Cardiac; drug effects; physiology; Patch-Clamp Techniques; Potassium Channels; drug effects; physiology
From: Chinese Journal of Applied Physiology 2002;18(2):155-158
CountryChina
Language:Chinese
Abstract:
AIM AND METHODSTo observe the effects of Puerarin on K+ channel of isolated ventricular myocyte in guinea pig.
METHODSUsing inside-out configuration of patch-clamp single channel recording technique.
RESULTSPuerarin 20 micromol/L, 40 micromol/L, 80 microml/L could inhibit the open-close rate of K+ channel of isolated ventricular myocyte in guinea pig. At 80 micromol/L, Po was decreased from 0.867 +/- 0.13 to 0.019 +/- 0.01 (n = 5, P < 0.05).
CONCLUSIONPuerarin can inhibit K+ channel of isolated ventricular myocyte in guinea pig. It may be the mechanism of Puerarin against arrhythmias in molecular level.