1, 25-Dihydroxyvitamin D3 increases in vitro vascular calcification through calcified blood vessel cells.
- Author:
Yu-Mei WANG
1
;
Shi-Wen WANG
;
Wan-Ren TONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcitriol; metabolism; Cattle; Cells, Cultured; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; drug effects; metabolism; pathology; Osteocalcin; metabolism; Vascular Calcification; metabolism; pathology; Vitamin D; analogs & derivatives; pharmacology
- From: Chinese Journal of Applied Physiology 2002;18(2):162-165
- CountryChina
- Language:Chinese
-
Abstract:
AIMAccumulated evidence suggest that the development of vascular calcification is similar to osteogenesis. Here we want to elucidate the effect of the common used osteo-regulatory factor 1,25(OH)2D3 on vascular calcification.
METHODS AND RESULTSAdding 10(-9) mol/L to the culture media 1,25(OH)2D3 time dependently increased the calcium deposition on the in vitro calcification of bovine vascular smooth muscle cells (BVSMCs) induced by beta-GP. It also increased cellular alkaline phosphatase activity by 301.1% during the calcified process. Osteocalcin, one of the osteogenic specific metric proteins, was dramatically elevated by 58.3% during the calcified processes, which indicate the transformation of BVSMCs to osteoblastic cell. 1,25(OH)2D3 had no such effect on non-calcified BVSMCs.
CONCLUSIONThese data suggest that 1,25(OH)2D3 exerts a stimulatory effect on vascular calcification through increasing the synthesis of ALP. This effect shares the same character as osteoblast cells. This effect is limited to the calcified prone vascular cell.
