TGF-beta and HSP70 expression in human skin fibroblasts after ultraviolet B irradiation.
- Author:
Yu CAO
1
;
Hao TANG
;
Hong-Yan ZHAO
;
Fan ZHANG
;
Yu YANG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Fibroblasts; metabolism; radiation effects; HSP70 Heat-Shock Proteins; metabolism; Humans; Skin; cytology; Transforming Growth Factor beta; metabolism; Ultraviolet Rays
- From: Chinese Journal of Applied Physiology 2002;18(2):166-168
- CountryChina
- Language:Chinese
-
Abstract:
AIM AND METHODSFor further understanding the signal transduction pathway of the inducible HSP70 expression the expression of TGF-betamRNA and HSP70 in fibroblasts after 5, 10 and 20 mJ/cm2 of UVB irradiation, and the content of TGF-beta protein in culture supernatants and HSP70 expression in fibroblasts by 10mJ/cm2 UVB irradiation after preincubation with the anti-human TGF-beta receptor II antibody were observed.
RESULTS(1) TGF-betamRNA expression positively correlated with HSP70 expression in UVB irradiation of different doses (r = 0. 906). (2) TGF-beta content in the culture supernatants negatively correlated with the HSP70 expression induced in adding anti-human TGF-beta receptor II antibody and UVB treatment condition (r = - 0.995).
CONCLUSIONTheTGF-beta play an important action in HSP70 inducing by UVB irradiation.
