Effects of cryopreservation length on the proliferative potential of cord blood hematopoietic cells in vitro.
- Author:
Mei-Ling ZHU
1
;
Ru-Guang CHEN
;
Yong-Zhi XI
;
Yong-Mei LIU
;
Li-Zhen PENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; Cell Survival; Cells, Cultured; Cryopreservation; methods; Fetal Blood; cytology; Hematopoietic Stem Cells; cytology; Humans; Time Factors
- From: Chinese Journal of Applied Physiology 2002;18(2):183-185
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effects of cryopreservation length on the proliferative potential of hematopoietic cells derived from cord blood.
METHODSUsing Dextran-40 and 10% DMSO as cryoprotectants, separated nuclear cells were stored in liquid nitrogen after they were freezed according programme. One month or 4 months later, they were thawed and expanded in serum-free medium for culture and expansion of hematopoietic cell (SFEM) for 5 weeks. Dynamic results were detected every week.
RESULTSAt the 5th week of expanding, TNC were expanded for 1499.0 +/- 115.6-folds and 1513.0 +/- 110.4-folds, respectively. CD34+ cells and CFCs reached to their highest level at the 2nd week and at the 3rd week. CD34+ cells were expanded for 63.8 +/- 6.1-folds and 62.4 +/- 5.7-folds, respectively. CFCs were expanded for 53.8 +/- 6.3-folds and 54.8 +/- 6.7-folds, respectively. Between the two kinds of cells, statistical significant difference in proliferative potential wasn't detected.
CONCLUSIONIn ideal cryopreservative condition, the cryopreservation length would do not affect the proliferative potential of cord blood hematopoietic cells.
