A simple and convenient method for assaying human RBCs glycolysis rate without oxygen.
- Author:
Xiu-Zhen LIU
1
;
En-Pu MA
;
Ying HAN
;
Xue NAN
;
Su-Ping REN
;
An LIU
;
Peng JIN
Author Information
- Publication Type:Journal Article
- MeSH: Erythrocytes; metabolism; physiology; Glycolysis; physiology; Hematologic Tests; methods; Humans; Oxygen; metabolism
- From: Chinese Journal of Applied Physiology 2002;18(2):190-192
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo convenience of the methods for assaying red blood cell glycolysis without oxygen condition in the studies.
METHODSReagent kit of glucose, perchloric acid, visible light prismatic photometer, battle of nitrogen and rocking bed are used in the studies. The process includes 4 steps prepare Tris- HCI solution and so on, assay of red blood cell glycolysis without oxygen condition and account of glycolysis rate.
RESULTSHuman red blood cells stored at 4 degrees C for 75 d, in SOD solution, the glycolysis rate is 86.2% +/- 5.0%, distinctly better than GMA solution (39.2% +/- 8.9%).
CONCLUSIONThe methods of assaying glycolysis without oxygen condition not use Habea's apparatus. The operation is convenient and simple and its determinations can be performed in ordinary laboratory and is is accurate.
