Zymography identification of the matrix metalloproteinases synthesized by cultured hepatic stellate cells.

Author: Wen-Xing ZHAO ¹ ; Chong-Li LIANG ; Bin ZHAO
Author Information

1. Medical Laboratory of Kunming General Hospital, Kunming 650032, China.
Publication Type:Journal Article
MeSH: Animals; Cells, Cultured; Electrophoresis, Polyacrylamide Gel; methods; Hepatic Stellate Cells; metabolism; Matrix Metalloproteinases; metabolism; Rats; Rats, Wistar
From: Chinese Journal of Applied Physiology 2002;18(2):193-195
CountryChina
Language:Chinese
Abstract:
AIMTo establish a method for identification of the activities of matrix metalloproteinases (MMPs) synthesized by cultured hepatic stellate cells (HSC).
METHODSSDS-gelatin polyacrylamide gels electrophoresis was used for the identification of MMPs synthesized by cultured HSC.
RESULTSMMP-2 and MMP-9 in culture medium and HSC were sensitively identified, and MMP-2 activity was higher than MMP-9 in culture medium, the changes of MMPs in culture medium and HSC were also observed after 24h medicine treatment.
CONCLUSIONZymography is suitable for the studies on the role of HSC in the regulation of extracellular matrix turnover.