Effects of anoxia/reoxygenation on Fos and Jun expression and apoptosis in cultured rat hippocampal neurons.
- Author:
Ai-Shi DING
1
;
Fu-Zhuang WANG
;
Li-Ying WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Cell Hypoxia; Cells, Cultured; Genes, fos; Genes, jun; Hippocampus; metabolism; Neurons; metabolism; Oxygen; metabolism; Rats; Rats, Wistar
- From: Chinese Journal of Applied Physiology 2002;18(3):213-217
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of anoxia/reoxygenation on Fos and Jun expression and apoptosis in cultured rat hippocampal neurons.
METHODSThe hippocampal neurons cultured for 12 d were exposed to anoxia environment (90% N2 + 10% CO2) for 4 h and then reoxygenated for 24 h and 72 h. The neurons were immunocytochemically stained using the antiserum against Fos and Jun, and the apoptosis were detected by using the terminal deoxynucleotidyl transferase mediated biotinylated deoxyuridine triphosphate nickel end labeling (TUNEL) method and flow cytometric analysis.
RESULTSThe percentage of Fos and Jun positive neurons and apoptosis neurons in cultured hippocampal neurons after anoxia/reoxygenation increased than those in control.
CONCLUSIONThe occurrence of neurons apoptosis is related to the increase in Fos and Jun expression in cultured hippocampal neurons after anoxia/reoxygenation.
