Effect of PDGF-AA on Ca2+i in vascular smooth muscle cells in SHRs.
- Author:
Jun JIN
1
;
Shan-Jun ZHU
;
Zhi-Ming ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Male; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; metabolism; Platelet-Derived Growth Factor; metabolism; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Receptors, Platelet-Derived Growth Factor; metabolism
- From: Chinese Journal of Applied Physiology 2002;18(3):274-277
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore the relationship between proliferation and hypertrophy of vascular smooth muscle cells and PDGF-AA and PDGFR-alpha expression in SHRs and the role of [Ca2+]i in it.
METHODSExpress difference of PDGF-AA, PDGFR-alpha, PDGFR-beta in SHR/WKY-VSMC was observed by Western blot. The effect of Ca2+ inhibitor (nimodipine) on proliferation, hypertrophy and free Ca2+ concentration of SHR-VSMC induced by PDGF-AA was observed by Western blot, [3H] incorporation and fluorescent digital image technique.
RESULTSPDGF-AA and PDGFR-alpha expression was markedly increased in SHR-VSMC than in WKY-VSMC, but PDGFR-beta was not different in SHR and WKY-VSMC. PDGF-AA-stimulated PCNA expression, [3H] incorporation and [Ca2+]i increasing were observed in SHR-VSMC. Dose-dependent nimodipine-inhibited PCNA expression, [3H] incorporation and [Ca2+]i increasing induced by PDGF-AA also were observed in SHR-VSMC.
CONCLUSIONSSpontaneously expression increasing of PDGF-AA and PDGFR-alpha in spontaneously hypertension rats (SHRs) may be one of the important factors on vascular reactivity and vascular modeling mediated through proliferation and hypertrophy in SHR-VSMC, and [Ca2+]i play an important role in this process.
