Cold stress induces the suppression of splenic NK cell activity and the c-fos expression in rat brain.
- Author:
Xing-hong JIANG
1
;
Shi-yu GUO
;
Shuang XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arginine Vasopressin; metabolism; Brain; metabolism; Cold Temperature; Killer Cells, Natural; metabolism; Male; Proto-Oncogene Proteins c-fos; metabolism; Rats; Rats, Wistar; Spleen; cytology; immunology; Stress, Physiological; Tyrosine 3-Monooxygenase; metabolism
- From: Chinese Journal of Applied Physiology 2002;18(4):313-316
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the effect of cold stress on the splenic NK cell activity and the c-fos expression in rat brain.
METHODSRats were maintained in cold chamber at 4 degrees for 4 h. The 51Cr release assay from YAC-1 cells was used to determine the splenic NK cell activity and the double staining of ABC method was employed to observe the immunoreactive expression of Fos, arginine-vasopressin and tyrosine hydroxylase.
RESULTSCold stress could induce a marked suppression of splenic NK cell activity and a significant expression of Fos protein in hypothalamic paraventricular nucleus(PVN), as well as in the brainstem locus coeruleus (LC). Double staining showed c-expression of Fos and arginine-vasopressin in some of PVN neurons, and co-expression of Fos and tyrosine hydroxylase in the majority of LC neurons.
CONCLUSIONThe vasopressinergic neurons in PVN and the catecholaminergic neurons in LC might be probably involved in the suppression of splenic NK cell activity induced by cold stress.
