All-trans retinoic acid enhances bystander effect of suicide-gene therapy against androgen-unresponsive prostate cancer.
- Author:
Wei-Guo CHEN
1
;
Chun-Yin YAN
;
Jian-Quan HOU
;
Duan-Gai WEN
;
Jin-Xian PU
;
Heng-Bing WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; pharmacology; Bystander Effect; drug effects; Cell Line, Tumor; Cell Survival; drug effects; Ganciclovir; pharmacology; Genes, Transgenic, Suicide; genetics; Genetic Therapy; methods; Humans; Male; Mice; Mice, Nude; Prostatic Neoplasms; genetics; pathology; therapy; Reverse Transcriptase Polymerase Chain Reaction; Simplexvirus; enzymology; Thymidine Kinase; genetics; metabolism; Tretinoin; pharmacology; Xenograft Model Antitumor Assays; methods
- From: National Journal of Andrology 2008;14(2):122-125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the enhancing effect of all-trans retinoic acid (ATRA) on the bystander effect of the herpes simplex virus thymidine kinase(HSV-TK)/ganciclovir (GCV) against androgen unresponsive prostate cancer.
METHODSThe bystander effect of the HSV-TK/GCV system was measured by methyl thiazolyl tetrazolium (MTT) assay on PC-3 cells before and after ATRA treatment. The growth and the histopathology of transplant tumors were observed in 4 groups of nude mice with prostate cancer.
RESULTSATRA augmented significantly the bystander effect of the HSV-TK/GCV system by reducing TK positive PC-3 cells from 50% to 30% (P < 0.05). HSV-TK showed an inhibiting effect, while ATRA with the HSV-TK/GCV system produced significant effect on prostate cancer 1 week earlier than the former (P < 0.05).
CONCLUSIONATRA can argument the in vivo and in vitro bystander effect of the HSV-TK/GCV system in the treatment of androgen unresponsive prostate cancer.
